Basic Science

Basic Science

The following Basic Science papers represent internal studies performed by AlloSource.

ProChondrix® CR

ProChondrix CRCryopreserved Osteochondral Allograft, ProChondrix® CR Maintains Metabolically Active, Viable Cells

ProChondrix CR maintains viable, metabolically active cells at 6-months of cryopreservation. Average cell viability was found to be 88.8%, and metabolic activity of the cells displayed cellular outgrowth capabilities within all cryopreserved samples.





ProChondrix Fresh Osteochondral Allograft Maintains Viable Chondrocytes, Osteoblasts and a Mineralized Matrix Necessary to Support Bone and Cartilage Formation

Quantitative and qualitative analysis revealed the presence of osteoblast progenitor cells and calcium deposition within the graft’s matrix, signifying that ProChondrix is conducive to osteoblast progenitor growth and differentiation as well as deep-layer mineralization.


ProChondrixProChondrix Cartilage Restoration Matrix Contains Growth Factors Necessary for Hyaline Cartilage Regeneration

Independently, marrow stimulation procedures do not promote excretion of growth factors or extracellular matrix proteins necessary for repair and regeneration of healthy hyaline cartilage, make it a short-term solution. This study examines the types and expression level of growth factors present in ProChondrix, and reviews their role in hyaline cartilage restoration and maintenance.


ProChondrixExtracellular Matrix and Growth Factors Expression in ProChondrix is Comparable to Unprocessed Adult Cartilage: A Rationale for Considering Signaling Dynamics

While ProChondrix has similar capabilities for chondrocyte outgrowth as compared to juvenile cartilage, the growth factor expression in ProChondrix more closely resembles that of healthy, unprocessed adult cartilage tissue, indicating a more relevant healing profile necessary to facilitate adult cartilage healing.


ProChondrixHigh Chondrocyte Viability of ProChondrix at the Maximum Shelf Life of 35 Days: Potential for Good Clinical Outcome and Consistency of Cartilage Allograft

Immunofluorescence staining revealed proliferating chondrocytes migrated out of the graft and into the surrounding fibrin glue, not only maintaining their proliferative capacity, but retaining their chondrocyte genotype as well. At the maximum shelf life of 35 days, ProChondrix demonstrated an average cell viability of 87.5%.